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        Ribonuclease A

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        Ribonuclease A

        EINECS 232-646-6
        CAS No. 9001-99-4 Density
        Solubility Melting Point 243 °C (dec.)
        Formula C9H14N4O3 Boiling Point
        Molecular Weight 226.23246 Flash Point
        Transport Information Appearance White to white with a yellow cast powder
        Safety 22-24/25 Risk Codes
        Molecular Structure Molecular Structure of 9001-99-4 (Ribonuclease A) Hazard Symbols
        Synonyms

        Ribonuclease

         

        Ribonuclease A Chemical Properties

        Molecular Formula: C9H14N4O3
        Molecular Weight: 226.2325
        EINECS: 207-332-7
        Density: 1.375 g/cm3
        Melting Point: 243 °C (dec.)
        Flash Point: 350.7 °C
        Boiling Point: 656.2 °C at 760 mmHg
        Vapour Pressure: 4.08E-18 mmHg at 25 °C 
        Enthalpy of Vaporization: 101.53 kJ/mol
        Storage temp.: 2-8 °C
        Structure of Ribonuclease A (CAS NO.9001-99-4):
                              
        IUPAC Name: 2-(3-Aminopropanoylamino)-3-(1H-imidazol-5-yl)propanoic acid
        Canonical SMILES: C1=C(NC=N1)CC(C(=O)O)NC(=O)CCN
        InChI: InChI=1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)
        InChIKey: CQOVPNPJLQNMDC-UHFFFAOYSA-N

        Ribonuclease A History

        The importance of bovine pancreatic Ribonuclease A (CAS NO.9001-99-4) was secured when the Armour & Co. (of hot dog fame) purified a kilogram of it, and gave 10 mg samples away free to any interested scientists. The ability to have a single lot of purified enzyme instantly made RNase the model system for protein studies.Ribonuclease-S, which is RNase A that has been treated with subtilisin, was the third protein to have its structure solved, in 1967. Studies of the oxidative folding of RNase A led Chris Anfinsen to enunciate the thermodynamic hypothesis of protein folding, which states that the folded form of a protein represents the minimum of its free energy.

        Ribonuclease A Uses

         Ribonuclease A (CAS NO.9001-99-4) is used for biochemical research.

        Ribonuclease A Production

         Ribonuclease A (CAS NO.9001-99-4) can be obtained from the fermentation broth of the grate produced by erythromycin.

        Ribonuclease A Toxicity Data With Reference

        Organism Test Type Route Reported Dose (Normalized Dose) Effect Source
        mouse LD50 intramuscular 355mg/kg (355mg/kg)   "Nukleazy: Biologicheskaya Rol i Prakticheskoe Ispol'zovanie. Nucleases: Biological Function and Practical Applications," Berdyshev, G.D., et al., eds., Kiev, USSR, Izdatel'stvo Naukova Dumka, 1985Vol. -, Pg. 56, 1985.
        mouse LD50 intraperitoneal 250mg/kg (250mg/kg)   "Nukleazy: Biologicheskaya Rol i Prakticheskoe Ispol'zovanie. Nucleases: Biological Function and Practical Applications," Berdyshev, G.D., et al., eds., Kiev, USSR, Izdatel'stvo Naukova Dumka, 1985Vol. -, Pg. 56, 1985.
        mouse LD50 subcutaneous 230mg/kg (230mg/kg)   "Nukleazy: Biologicheskaya Rol i Prakticheskoe Ispol'zovanie. Nucleases: Biological Function and Practical Applications," Berdyshev, G.D., et al., eds., Kiev, USSR, Izdatel'stvo Naukova Dumka, 1985Vol. -, Pg. 56, 1985.
        rat LD50 intramuscular 310mg/kg (310mg/kg)   "Nukleazy: Biologicheskaya Rol i Prakticheskoe Ispol'zovanie. Nucleases: Biological Function and Practical Applications," Berdyshev, G.D., et al., eds., Kiev, USSR, Izdatel'stvo Naukova Dumka, 1985Vol. -, Pg. 56, 1985.
        rat LD50 intraperitoneal 392mg/kg (392mg/kg)   "Nukleazy: Biologicheskaya Rol i Prakticheskoe Ispol'zovanie. Nucleases: Biological Function and Practical Applications," Berdyshev, G.D., et al., eds., Kiev, USSR, Izdatel'stvo Naukova Dumka, 1985Vol. -, Pg. 56, 1985.
        rat LD50 subcutaneous 290mg/kg (290mg/kg)   "Nukleazy: Biologicheskaya Rol i Prakticheskoe Ispol'zovanie. Nucleases: Biological Function and Practical Applications," Berdyshev, G.D., et al., eds., Kiev, USSR, Izdatel'stvo Naukova Dumka, 1985Vol. -, Pg. 56, 1985.

        Ribonuclease A Safety Profile

        Safety Statements:
        S22:Do not breathe dust. 
        S24/25:Avoid contact with skin and eyes.
        WGK Germany: 3
        RTECS: RF0760000
        F 10: Keep under argon.
        F 21: Sensitive to humidity.

        Ribonuclease A Specification

         Ribonuclease A , its cas register number is 9001-99-4. It also can be called Alkaline ribonuclease ; Amphinase ; Pancreatic ribonuclease ; RNase; Ribonuclease ; S-RNase ; and Ribonucleic phosphatase .

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